Immunopeptidomics:Article Title: Spatial characterisation of TREM2 expression in actively demyelinating multiple sclerosis lesions supports its key roles in lipid metabolic pathways.
Article Snippet: .. Goat anti-human TREM2 (Polyclonal) Microglia/ macrophages Endothelium R&D Systems, USA (AF1828) 1:200 Rabbit anti-human Iba1/AIF1 (Polyclonal) Microglia/ macrophages Wako, Japan (019-19741) 1:100 Mouse anti-human CD163 (Monoclonal) (Perivascular) macrophages/rare microglia Abcam, UK (ab201461) 1:100 Mouse anti-human CD68 (Monoclonal) Microglia/ macrophages Agilent, USA (M087601-2) 1:75 Mouse anti-human MHC class II (Monoclonal) Microglia/ macrophages Invitrogen, USA (MA1-25914) 1:50 Rabbit anti-human ADRP/Perilipin 2 (Polyclonal) Lipid Droplet Storage United Bioresearch, Australia (15294-1-AP) 1:50 Rabbit anti-human MS4A4A (Polyclonal) Macrophages/rare microglia Novus, USA (NBP1-86542) 1:100 Rabbit anti-human TMEM119 (Polyclonal) Microglia Sigma-Aldrich, USA (HPA051870) 1:1000 Rat anti-human/mouse TREM2 (Monoclonal) Nonspecific R&D Systems, USA (MAB17291) 1:200 Mouse anti-human Iba1 (Monoclonal) Microglia Sigma-Aldrich, USA (MABN92) 1:50 Rabbit anti-human TREM2 (Polyclonal) Nonspecific Cusabio, US (CSBPA024405LA01HU) 1:100 Rabbit anti-human TREM2 (Polyclonal) Nonspecific ProSci, USA (13-679) 1:100 Rabbit anti-human TREM2 (Polyclonal) Endothelium Invitrogen, USA (PA5-87933) 1:100 Rabbit anti-human TREM2 (Monoclonal) Nonspecific Cell Signalling, USA (D8I4C) 1:100 Mouse anti-human TREM2 Microglia N/A (21E10) 1:200 Mouse anti-human TREM2 Microglia N/A (T22) 1:200 Goat IgG Control Non-immune control R&D Systems, USA (AB-108-C) .. Conjugate) Reactivity Source (Cat#) Dilution Donkey anti-rabbit (AF647) Anti-rabbit primary antibodies Invitrogen, USA (A-32795) 1:200 Donkey anti-goat (AF555) Anti-goat primary antibodies Invitrogen, USA (A-21432) 1:200 Donkey anti-mouse (AF488) Anti-mouse primary antibodies Invitrogen, USA (A-32766) 1:200 Table 3.
Control:Article Title: Spatial characterisation of TREM2 expression in actively demyelinating multiple sclerosis lesions supports its key roles in lipid metabolic pathways.
Article Snippet: .. Goat anti-human TREM2 (Polyclonal) Microglia/ macrophages Endothelium R&D Systems, USA (AF1828) 1:200 Rabbit anti-human Iba1/AIF1 (Polyclonal) Microglia/ macrophages Wako, Japan (019-19741) 1:100 Mouse anti-human CD163 (Monoclonal) (Perivascular) macrophages/rare microglia Abcam, UK (ab201461) 1:100 Mouse anti-human CD68 (Monoclonal) Microglia/ macrophages Agilent, USA (M087601-2) 1:75 Mouse anti-human MHC class II (Monoclonal) Microglia/ macrophages Invitrogen, USA (MA1-25914) 1:50 Rabbit anti-human ADRP/Perilipin 2 (Polyclonal) Lipid Droplet Storage United Bioresearch, Australia (15294-1-AP) 1:50 Rabbit anti-human MS4A4A (Polyclonal) Macrophages/rare microglia Novus, USA (NBP1-86542) 1:100 Rabbit anti-human TMEM119 (Polyclonal) Microglia Sigma-Aldrich, USA (HPA051870) 1:1000 Rat anti-human/mouse TREM2 (Monoclonal) Nonspecific R&D Systems, USA (MAB17291) 1:200 Mouse anti-human Iba1 (Monoclonal) Microglia Sigma-Aldrich, USA (MABN92) 1:50 Rabbit anti-human TREM2 (Polyclonal) Nonspecific Cusabio, US (CSBPA024405LA01HU) 1:100 Rabbit anti-human TREM2 (Polyclonal) Nonspecific ProSci, USA (13-679) 1:100 Rabbit anti-human TREM2 (Polyclonal) Endothelium Invitrogen, USA (PA5-87933) 1:100 Rabbit anti-human TREM2 (Monoclonal) Nonspecific Cell Signalling, USA (D8I4C) 1:100 Mouse anti-human TREM2 Microglia N/A (21E10) 1:200 Mouse anti-human TREM2 Microglia N/A (T22) 1:200 Goat IgG Control Non-immune control R&D Systems, USA (AB-108-C) .. Conjugate) Reactivity Source (Cat#) Dilution Donkey anti-rabbit (AF647) Anti-rabbit primary antibodies Invitrogen, USA (A-32795) 1:200 Donkey anti-goat (AF555) Anti-goat primary antibodies Invitrogen, USA (A-21432) 1:200 Donkey anti-mouse (AF488) Anti-mouse primary antibodies Invitrogen, USA (A-32766) 1:200 Table 3.
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